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ISSN : 1225-5009(Print)
ISSN : 2287-772X(Online)
Flower Research Journal Vol.32 No.S pp.16-16

Exploring Polyploid Induction in Lilium Using Chemical Agents in Tissue Culture

Ji-Yun Kang1, Eun-Jae Seo1, Moon-Seok Kang1, Yun-Jae Ahn1,2, Ki-Byung Lim1,2*
1Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea
2Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Korea


The potential for inducing polyploidy in Lilium species is substantial for enhancing ornamental traits and genetic diversity. To examine the efficacy of colchicine, oryzalin, and caffeine in stimulating polyploidy through bulb-scale tissue culture, this study was conducted. Varying concentrations of these chemical agents were employed to assess their impact on polyploid induction. Concentrations of colchicine (0.05%, 0.1%, 0.2%), oryzalin (0.001%, 0.003%, 0.005%), and caffeine (0.6%, 0.9%, 1.2%) were applied for 3 hours, followed by washing with deionized water and streaking onto half MS medium. The half-MS medium consisted of 30 g sucrose, 2.2 g MS, 4 g gelrite, and a pH of 5.8. Observations were made in the dark culture. Interestingly, colchicine treatment resulted in lethality, suggesting potential challenges with this method. However, the use of oryzalin (0.001%, 0.003%) and caffeine (0.6%, 0.9%) at specific concentrations yielded promising results, successfully stimulating callus differentiation. These findings shed light on the complex mechanisms underlying polyploid induction in Lilium and pave the way for refining protocols to optimize polyploid breeding strategies in ornamental plants. Polyploidy induction enables the rapid generation of genetic variation to develop new traits and characteristics. Further cytologic studies will be conducted to confirm the polyploidy.




  2. Journal Abbreviation : 'Flower Res. J.'
    Frequency : Quarterly
    Doi Prefix : 10.11623/frj.
    ISSN : 1225-5009 (Print) / 2287-772X (Online)
    Year of Launching : 1991
    Publisher : The Korean Society for Floricultural Science
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