Cymbidium ‘Lovely King’ was developed as a new hybrid in the National Institute of Horticultural & Herbal Science, it has a potential for important cut flower because of its various colors, shapes, and longevity. Stem blockage by microbial growth or physiological plugging causes decreasing water uptake and vase life. Nanobubble is a small gas bubble in liquids with 0.2 mm in diameter. Oxygen nanobubble (NB) can replenish dissolved oxygen (DO) used for inactive microorganisms in the vase solution. Ozone (O3) is used as an antimicrobial due to its sterilization ability. This study was conducted to investigate the vase life, water uptake, relative fresh weight (RFW), petal color, and the number of bacteria of cut Cymbidium ‘Lovely King’ under various levels of NB and O3NB. The O3 and DO concentrations were 0.0 and 11.6 mg･L-1 (TW, control), 0.0 and 19.2 mg･L-1 (NB 20%), 0.0 and 23.2 mg･L-1 (NB 33%), 0.0 and 32.0 mg･L-1 (NB 50%), 0.0 and 51.6 mg･L-1 (NB 100%), 2.1 and 20.6 mg･L-1 (O3NB 20%), 3.1 and 24.1 mg･L-1 (O3NB 33%), 4.6 and 39.5 mg･L-1 (O3NB 50%) and 6.0 and 52.9 mg･L-1 (O3NB 100%), respectively. The vase life was increased 9 days more in C. ‘Lovely King’ treated with O3NB 20% compared to 20 days of the control. The water uptake was 6.9 g in C. ‘Lovely King’ treated with O3NB 20% on day 4; however, it ranged from 4.9 to 6.5 g in other treatments. The RFW was increased in C. ‘Lovely King’ treated with O3NB 20% than the control from days 4 to 20 except for days 5 and 7. The L* and b* values were lower in C. ‘Lovely King’ treated with O3NB 33% and O3NB 20% than the control during the latter of experimental periods, respectively, meaning that the senescence of petal was delayed in C. ‘Lovely King’ treated with those treatments. The bacterial count was lower in C. ‘Lovely King’ in all NB and O3NB treatments than in control. This result indicated that the postharvest quality improved when NB and O3 were combined in the vase solution at a range of O3NB 20% in Cymbidium ‘Lovely King’.