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ISSN : 1225-5009(Print)
ISSN : 2287-772X(Online)
Flower Research Journal Vol.30 No.S pp.9-9
DOI : https://doi.org/10.11623/frj.2022.30.S.9

Change in the Survival, Quality, and Nutrient Uptake Response of In Vitro Gerbera Plantlets According to Low-Temperature Storage Duration

Ho Jin Lee1, Je Yeon Yeon2, Min Ji Kim1, Yeong Chan Shin1, Wan Soon Kim1,2*
1Department of Environmental Horticulture, University of Seoul, Seoul 02504, Korea
2Natural Science Institute, University of Seoul, Seoul 02504, Korea

*Corresponding author: Wan Soon Kim
Tel. 02-6409-5613, E-mail:

Abstract

This study was carried out to find out the long-term storage conditions for healthy growth after acclimatization while minimizing subculture in gerbera tissue culture. We investigated the quality and uptake response of the plantlets including growth, root activity, chlorosis ratio, and survival rate after storage for 3 to 48 weeks through the alternate treatment of the tissue culture plantlets at storage temperatures (4 or 8℃) with or without light (light of 30 μmol･m-2s-1 or darkness). Up to 12 weeks, all plantlets survived 90% or more regardless of treatment conditions. The uptake of mineral nutrients in low-temperature conditions decreased as the storage period increased, and most of them were found to be large in the darkness conditions. At 4℃ with light they survived 100% maintaining a slow growth, so it was the most suitable storage environment. After 24 weeks, however, the survival rate decreased sharply to 74% in light conditions and 29% in darkness conditions. 48 weeks later, all withered with or without light. Thus, 4℃ was not suitable as a long-term storage environment. In the meantime, at 8℃, their biomass including plant height, number of leaves and dry weight increased with light, so in vitro growth progressed, but their 36 weeks survival rate was 100%. The chlorosis ratio of the plants was 21% higher in treatment in the darkness condition than in the light condition. This result means that the minimal light source and temperature are necessary for the long-term storage for 12 weeks or more.

초록

 

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  2. Journal Abbreviation : 'Flower Res. J.'
    Frequency : Quarterly
    Doi Prefix : 10.11623/frj.
    ISSN : 1225-5009 (Print) / 2287-772X (Online)
    Year of Launching : 1991
    Publisher : The Korean Society for Floricultural Science
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